Washington University of St. Louis Gastroenterology in St. Louis, Missouri

Post Doc Instructions

Please do not submit an application to this posting. To apply for this postdoctoral research opportunity, follow the directions below in “Applicant Special Instructions”.

Job Type/Schedule

This position is full-time with approximately 40 hours per week. Hours are generally normal business hours but may vary due to the timing for experiments and other research activity deadlines.

Department Name/Job Location

This position is in the Department of Medicine, Division of Gastroenterology. The position is for the Medical School Campus.

Essential Functions

POSITION SUMMARY:

We are studying the genetics, etiology, and pathobiology of colon cancer, with a particular focus on miRNAs, transcription factors, and their target genes/mRNAs. Many of the pathways that drive stem cell fate/survival in the intestine are also potentially oncogenic; this compels our research into the biology of intestinal stem cells (ISCs) – what pathways are involved in ISC establishment? What drives ISC fate? What controls ISC census? We have recently described how the tumor suppressor miRNA family Let-7 is critical for suppressing the expression of mRNAs that drive both ISC fate and intestinal tumorigenesis. We are also interested in probing the functional integration of miRNA targets, such as those targeted and repressed by Let-7. These targets may cooperatively integrate an oncogenic signal, and using novel approaches we hope to probe their combinatorial effects on intestinal tumorigenesis. Our studies focus on the temporal extremes of carcinogenesis: the initial genetic lesions and micro-environmental millieux that trigger pre-cancerous changes and the late events of dissemination and metastasis.

We use genetically engineered mouse models (GEMMs) and organoids to study these processes, including traditional Cre-Lox conditional/tissue-specific mutagenesis approaches and next generation genome editing tools, with a specific focus on CRISPR/Cas site-specific nucleases and the Piggybac DNA transposon. The development of new GEMMs will be accomplished using standard techniques (molecular cloning, recombineering, pro-nuclear injection, homologous recombination), Piggybac, and CRISPR/Cas. In addition to mouse models, we employ ex vivo organoid cultures to probe mechanisms of growth, proliferation, stem cell fate, the micro-environment, and lineage allocation of epithelial cells. Molecular investigations will be pursued using RNP-CLIP-Seq (HITS-CLIP), mRNA/miRNA-seq, ChIP-seq, novel reporter assays, quantitative RT-PCR, PCR, immunoblots, DNA cloning, and standard molecular biology and biochemical assays.

Required Qualifications

  • Applicants must hold a doctorate (M.D., Ph.D., or equivalent) and be eligible to work in the U.S. at the time of employment.
  • Experience with techniques in molecular biology, cell biology, biochemistry, and vertebrate animal models is essential.
  • Good oral and written communication skills are required.
  • Applicants must be able to perform the work described and wear protective equipment when necessary.

Preferred Qualifications

  • Candidates with experience studying animal models (GEMMs), cancer biology, genetics, cell culture models, organoid/ex vivo culture, miRNAs, ncRNAs, and/or RNA-binding proteins are encouraged to apply.
  • Experience with recombinant DNA technology, transgenic/knockout mice, molecular biology, and molecular genetics is also preferred.
  • Candidates should be familiar or proficient with experimental approaches described above.
  • Candidates must also be able to carry out cell culture experiments, and handle, monitor, and breed mice, with competency performing dissections, injections, tissue processing, immunostaining, and microscopy.
  • Candidates should be able to work independently and have troubleshooting skills, with critical, analytical, and creative thinking.
  • Good documentation and organized record-keeping is also essential.
  • Most importantly, you must love science!

Salary Range

Base pay commensurate with experience.

Department Summary

The Division of Gastroenterology at the Washington University School of Medicine represents one of the finest examples of an academic training program, dedicated to the study of digestive and liver diseases. The balance of outstanding faculty, exemplary facilities, and a cohesive philosophy ensure consistent excellence across the missions of maintaining national prominence in research, teaching and patient care.

Benefits

This position is eligible for full-time benefits. Please visit our website at http://hr.wustl.edu to view a summary of benefits.

Applicant Special Instructions

Please send a C.V., cover letter, and list of 3-5 references to Dr. Blair Madison at bmadison@wustl.edu.

EOE Statement

Washington University is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, age, sex, sexual orientation, gender identity or expression, national origin, genetic information, disability, or protected veteran status.

Job Title: Postdoctoral Research Associate - Gastroenterology - 41510

Job ID: 41510

Location: Medical School

Full/Part Time: Full-Time

Regular/Temporary: Regular